Solutions

Cell culture technology

Regenerative therapy is a state-of-the-art medical treatment, but the culturing of cells relies on old processes, developed 100 years ago. As a result, only small amounts of product are available and they are extremely expensive. We are developing a highly efficient cell culturing system based on our cell fiber technology to solve this problem.
 

Support tool for biopharmaceutical development

In drug development, numerous animal experiments are necessary to evaluate the safety and efficacy of a medicine. However, it is difficult to predict all side-effects of a medicine before practical application because the responses in humans and experimental animals are different. Moreover, there are many ethical concerns surrounding animal experiments. To solve these problems, human iPS-derived cells have gained attention as a possible substitute to animal experiments and for dramatically improving the efficiency of the drug development process. We are developing a support tool for biopharmaceutical development that could solve the current problems of iPS-derived cell-based artificial tissue, such as insufficient functionality and instability in the manufacturing and preservation process.
 

Technology platform

Cell fiber is a technology that enables the handling of cells as if they were machine parts. It is developed by combining expertise in various fields, such as machine engineering, biology, chemistry, and materials science.
 
We are improving the cell fiber technology platform for industrial application and have developed a device that enables the mass production of cell fibers.
 

Paper

  • Kazuhiro Ikeda, Shogo Nagata, Teru Okitsu, and Shoji Takeuchi: Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells, Scientific Reports vol. 7, 2850, 2017
  • Minghao Nie and Shoji Takeuchi: Microfluidics based synthesis of coiled hydrogel microfibers with flexible shape and dimension control, Sensors & Actuators: B. Chemical, vol. 246, pp. 358–362, 2017
  • Fumisato Ozawa, Teru Okitsu, and Shoji Takeuchi: Improvement in Mechanical Properties of Cell-Laden Hydrogel Microfibers Using Interpenetrating Polymer Network, ACS Biomaterials Science & Engineering, vol. 3 (3), pp. 392–398, 2017
  • Hiroaki Onoe, Midori Kato-Negishi, Akane Itou and Shoji Takeuchi: Differentiation Induction of Mouse Neural Stem Cells in Hydrogel Tubular Microenvironments with Controlled Tube Dimensions, Advanced Healthcare Materials, vol. 5(9), pp. 1104–1111, 2016
  • Amy Y. Hsiao, Teru Okitsu, Hiroki Teramae, and Shoji Takeuchi: 3D Tissue Formation of Unilocular Adipocytes in Hydrogel Microfibers, Advanced Healthcare Materials, vol. 5(5), pp. 548–556, 2016
  • Yuya Morimoto, Amy Y. Hsiao, and Shoji Takeuchi: Point, Line and Plane-shaped Cellular Constructs for 3D Tissue Assembly, Advanced Drug Delivery Reviews, vol. 95, pp. 29-39, 2015 (review paper)
  • Keiko Sugai, Soraya Nishimura, Midori Kato-Negishi, Hiroaki Onoe, Shintaroh Iwanaga, Yoshiaki Toyama, Morio Matsumoto, Shoji Takeuchi, Hideyuki Okano, and Masaya Nakamura: Neural Stem/Progenitor Cell-Laden Microfibers Promote Transplant Survival in a Mouse Transected Spinal Cord Injury Model, Journal of Neuroscience Research, vol. 93(12), pp. 1826–1838, 2015
  • Soichiro Tottori and Shoji Takeuchi: Formation of liquid rope coils in a coaxial microfluidic device, RSC Advances, vol. 5, pp. 33691-33695, 2015
  • Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi: Smooth Muscle-like Tissue Constructs with Circumferentially Oriented Cells Formed by the Cell Fiber Technology, Plos ONE, 10(3):e0119010, 2015
  • Hiroaki Onoe and Shoji Takeuchi: Cell-laden microfibers for bottom-up tissue engineering, Drug Discovery Today, vol. 20(2), pp. 236–246, 2015 (review paper)
  • Hiroaki Onoe, Teru Okitsu, Akane Itou, Midori Kato-Negishi, Riho Gojo, Daisuke Kiriya, Koji Sato, Shigenori Mirua, Shintaroh Iwanaga, Kaori Kuribayashi-Shigetomi, Yukiko Matsunaga, Yuto Shimoyama, and Shoji Takeuchi: Metre-long Cell-laden Microfibres Exhibit Tissue Morphologies and Functions, Nature Materials, vol.12, pp. 584–590, 2013 , highlighted in LOC
  • Kayoko Hirayama, Teru Okitsu, Hiroki Teramae, Daisuke Kiriya, Hiroaki Onoe and Shoji Takeuchi: Cellular building unit integrated with microstrand-shaped bacterial cellulose, Biomaterials, vol. 34, pp. 2421-2427, 2013