CellFiber Co. Ltd.

SOLUTIONS & TECHNOLOGY

細胞医薬品の製造で困っていませんか

Spheroid

Heterogeneous sized cell aggregates Variation in quality

Adherent cells

Low harvest rate from microcarrier Hard to remove debris

Floating cells

Lower proliferation rate in shaking-culture

We can solve with CellFiberTM technology

 

What is CellFiberTM?

CellFiberTM is hollow gel tube with cells inside.

The diameter is few hundreds micrometer long.

Since both edges are closed, cells stay inside the fiber.

Substances produced by cells can be harvested through permeable gel.

Homogeneous cell aggregate

・Avoid necrosis in the center

・Mass-culturing in homogeneous environment

Dissolvable gel

・Remove after culturing

・ECM can be encapsulated

Protection

・Reduce damage from stirring.

・Permeable to: oxygen, nutrients, etc

Our Solutions

お使いの細胞に対する培養プロセス開発を行います。
研究用から臨床用まで、幅広い用途・細胞種に対応いたします。
お気軽にお問い合わせください。

お使いの細胞に対する
培養プロセス開発を行います。研究用から臨床用まで、幅広い
用途・細胞種に対応いたします。
お気軽にお問い合わせください。

装置提供

Equipment Provision
 

まずは小スケールで試してみたい、という

お客様向けに、ご自身で細胞ファイバを

作製できる装置を提供いたします。

ファイバ培養の利便性を実感ください。

共同研究

Joint Research
 

細胞をご提供いただき、お客様と二人三脚で

オーダーメイドの培養プロセスを開発します。

ライセンシング

Licensing
 

ファイバ培養で生産された製品を上市される

場合は、ランニングロイヤリティ形式での

ライセンスが可能です。

PUBLICATIONS

Kazuhiro Ikeda, Shogo Nagata, Teru Okitsu, and Shoji Takeuchi: Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells, Scientific Reports vol. 7, 2850, 2017

Minghao Nie and Shoji Takeuchi: Microfluidics based synthesis of coiled hydrogel microfibers with flexible shape and dimension control, Sensors & Actuators: B. Chemical, vol. 246, pp. 358–362, 2017

Fumisato Ozawa, Teru Okitsu, and Shoji Takeuchi: Improvement in Mechanical Properties of Cell-Laden Hydrogel Microfibers Using Interpenetrating Polymer Network, ACS Biomaterials Science & Engineering, vol. 3 (3), pp. 392–398, 2017

Hiroaki Onoe, Midori Kato-Negishi, Akane Itou and Shoji Takeuchi: Differentiation Induction of Mouse Neural Stem Cells in Hydrogel Tubular Microenvironments with Controlled Tube Dimensions, Advanced Healthcare Materials, vol. 5(9), pp. 1104–1111, 2016

Amy Y. Hsiao, Teru Okitsu, Hiroki Teramae, and Shoji Takeuchi: 3D Tissue Formation of Unilocular Adipocytes in Hydrogel Microfibers, Advanced Healthcare Materials, vol. 5(5), pp. 548–556, 2016

Yuya Morimoto, Amy Y. Hsiao, and Shoji Takeuchi: Point, Line and Plane-shaped Cellular Constructs for 3D Tissue Assembly, Advanced Drug Delivery Reviews, vol. 95, pp. 29-39, 2015 (review paper)

Keiko Sugai, Soraya Nishimura, Midori Kato-Negishi, Hiroaki Onoe, Shintaroh Iwanaga, Yoshiaki Toyama, Morio Matsumoto, Shoji Takeuchi, Hideyuki Okano, and Masaya Nakamura: Neural Stem/Progenitor Cell-Laden Microfibers Promote Transplant Survival in a Mouse Transected Spinal Cord Injury Model, Journal of Neuroscience Research, vol. 93(12), pp. 1826–1838, 2015

Soichiro Tottori and Shoji Takeuchi: Formation of liquid rope coils in a coaxial microfluidic device, RSC Advances, vol. 5, pp. 33691-33695, 2015

Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi: Smooth Muscle-like Tissue Constructs with Circumferentially Oriented Cells Formed by the Cell Fiber Technology, Plos ONE, 10(3):e0119010, 2015

Hiroaki Onoe and Shoji Takeuchi: Cell-laden microfibers for bottom-up tissue engineering, Drug Discovery Today, vol. 20(2), pp. 236–246, 2015 (review paper)

Hiroaki Onoe, Teru Okitsu, Akane Itou, Midori Kato-Negishi, Riho Gojo, Daisuke Kiriya, Koji Sato, Shigenori Mirua, Shintaroh Iwanaga, Kaori Kuribayashi-Shigetomi, Yukiko Matsunaga, Yuto Shimoyama, and Shoji Takeuchi: Metre-long Cell-laden Microfibres Exhibit Tissue Morphologies and Functions, Nature Materials, vol.12, pp. 584–590, 2013 , highlighted in LOC

Kayoko Hirayama, Teru Okitsu, Hiroki Teramae, Daisuke Kiriya, Hiroaki Onoe and Shoji Takeuchi: Cellular building unit integrated with microstrand-shaped bacterial cellulose, Biomaterials, vol. 34, pp. 2421-2427, 2013